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Summary: Chloroplast PPR protein: Required for expression of one or more chloroplast genes required for chloroplast translation. Null alleles of ppr2 are albino and seedling lethal due to the loss of all plastid-encoded proteins.
Images: mutant leaf color is #2 on the seedling color scale
First reported: Williams and Barkan 2003.
Key Alleles
ppr2-1::Mu and ppr2-4::Mu. Both have Mu insertions in the second exon. Both have an ivory, seedling lethal phenotype. Mutant seedlings tend to be smaller than their normal siblings.
Map Location
The location of ppr2 is inferred from near identity of Ppr2-B73 sequence with various probes mapped by recombination strategies: IDP1402 (Fu et al. 2006), PZA02035 (McMullen et al. 2009); or direct hybridization to the physical map: cl15341_1_ov (Gardiner et al. 2004).
Gene Product
A pentatricopeptide repeat (PPR) protein, located in the chloroplast stroma.
Expression
ppr2 mRNA overaccumulates in iojap1 mutant leaf tissue.
References
Fu Y, Wen T, Ronin Y, Chen HD, Guo L, Mester D, Yang Y, Lee M, Korol AB, Ashlock D, Schnable P (2006) Genetic dissection of intermated recombinant inbred lines using a new genetic map of maize. Genetics 174:1671-1683. PUBMED
Gardiner J, Schroeder S, Polacco ML, Sanchez-Villeda H, Fang Z, Morgante M, Landewe T, Fengler K, Useche F, Hanafey M, Tingey S, Chou H, Wing R, Soderlund C, Coe EH Jr (2004) Anchoring 9,371 maize expressed sequence tagged unigenes to the bacterial artificial chromosome contig map by two-dimensional overgo hybridization. Plant Physiol 134:1317-1326. PUBMED
McMullen MD, Kresovich S, Villeda HS, Bradbury P, Li H, Sun Q, Flint-Garcia S, Thornsberry J, Acharya C, Bottoms C, Brown P, Browne C, Eller M, Guill K, Harjes C, Kroon D, Lepak N, Mitchell SE, Peterson B, Pressoir G, Romero S, Oropeza Rosas M, Salvo S, Yates H, Hanson M, Jones E, Smith S, Glaubitz JC, Goodman M, Ware D, Holland JB, Buckler ES (2009) Genetic properties of the maize nested association mapping population. Science 325:737-740. PUBMED
Williams PM and Barkan A. (2003) A chloroplast-localized PPR protein required for plastid ribosome accumulation. Plant J 36:675-686. PUBMED
Links: MGDB ppr2 NCBI:ppr2 UniProt: Q7Y1W6