Maize Genetics Cooperation Newsletter

maize gene review

Author: Alice Barkan, University of Oregon, Eugene, OR. USA 97403


Review: pending

Pub date: May 2009

Name: ppr10-- pentricopeptide repeat 10

Chromosome 6L bin 6.02
Genome Browser via BAC c0144P08 sequence AC187904

Function: atpH and psaJ mRNA stabilization

image of corn mutant

Summary: Encodes a chloroplast PPR protein that binds to and stabilizes atpH and psaJ mRNAs. Null alleles of ppr10 condition very pale yellow green, non-photosynthetic and seedling lethal due to reduced accumulation of multiple photosynthetic enzyme complexes.

Image: mutant leaf color depicted on a seedling color scale

First reported: Pfalz et al. 2009.

Key Alleles

ppr10-1::MuDR; MuDR insertion in the exon. Very pale yellow green, seedling lethal phenotype.

ppr10-2::MuDR; MuDR insertion in the exon. Very pale yellow green, seedling lethal phenotype.

Map Location

Determined by sequence alignment to version 1 of the B73 genome sequence.

Gene Product

An RNA binding, PPR protein localized to chloroplast stroma. It is required for accumulation of atpH and psaJ mRNA and any RNA with a 5' or 3'-end mapping to these intergenic regions: atpI-atpH or psaJ-rpl33. It binds with specificity, both in vitro and in vivo, to a defined consensus mRNA sequence in these regions, protecting the mRNA from 5' and 3'-exonuclease degradation.

Related loci

atpH, psaJ; transcript stabilization


Pfalz J, Bayraktar OA, Prikryl J, Barkan A (2009) Site-specific binding of a PPR protein defines and stabilizes 5' and 3' mRNA termini in chloroplasts. EMBO J, in press. PUBMED

Links: MGDB ppr10 NCBI: FJ490677 UniProt: B8Y6I0