 |
Summary: Encodes a chloroplast PPR protein that binds to and stabilizes atpH and psaJ mRNAs. Null alleles of ppr10 condition very pale yellow green, non-photosynthetic and seedling lethal due to reduced accumulation of multiple photosynthetic enzyme complexes.
Image: mutant leaf color depicted on a seedling color scale
First reported: Pfalz et al. 2009.
Key Alleles
ppr10-1::MuDR; MuDR insertion in the exon. Very pale yellow green, seedling lethal phenotype.
ppr10-2::MuDR; MuDR insertion in the exon. Very pale yellow green, seedling lethal phenotype.
Map Location
Determined by sequence alignment to version 1 of the B73 genome sequence.
Gene Product
An RNA binding, PPR protein localized to chloroplast stroma. It is required for accumulation of atpH and psaJ mRNA and any RNA with a 5' or 3'-end mapping to these intergenic regions: atpI-atpH or psaJ-rpl33. It binds with specificity, both in vitro and in vivo, to a defined consensus mRNA sequence in these regions, protecting the mRNA from 5' and 3'-exonuclease degradation.
Related loci
atpH, psaJ; transcript stabilization
References
Pfalz J, Bayraktar OA, Prikryl J, Barkan A (2009) Site-specific binding of a PPR protein defines and stabilizes 5' and 3' mRNA termini in chloroplasts. EMBO J, in press. PUBMED