Maize Genetics Cooperation Newsletter

maize gene review

Author: Alice Barkan, University of Oregon, Eugene, OR. USA 97403

DOI/

Review: pending

Pub date: May 2009

Name: crs4--chloroplast RNA splicing4

Synonyms: ppr7, pentatricopeptide repeat7

Chromosome--not yet known-- cDNA sequence FL033043 aligns to 2 BACs on different chromosomes (May 2009)

Function: splicing of ndhA and ndhB.

image of corn mutant

Summary: A chloroplast PPR protein required for ndhA and ndhB splicing. Null alleles of crs4 condition pale yellow green, non-photosynthetic and seedling lethal due to reduced accumulation of photosynthetic enzyme complexes.

Images: left: mutant leaf color depicted on a seedling leaf color scale; right: Northern blot from ndhB; lower cartoon shows sites of Mu insertions.

First reported: Unpublished.

Key Alleles

crs4-1::Mu; Mu insertion is in the first exon. Pale yellow green, seedling lethal phenotype.

crs4-2::Mu; Mu insertion in the second exon. Pale yellow green, seedling lethal phenotype.

crs4-1/crs4-2 individuals are also pale yellow green, seedling lethal phenotype.

Map Location

Currently ambiguous. A cDNA sequence aligns to distinct regions on chromosomes 2 and 9.

Gene Product

A pentatricopeptide repeat protein required for ndhA and ndhB splicing.

References

Alice Barkan (unpublished).

Links: MGDB: crs4 NCBI: FL033043